The worth of creating extensive, tissue-particular maps of protein lysine acetylation sites is further underscored by the numerous role of posttranslational modifications within the evolution and phenotypic composition of vertebrates, as indicated by the significant selective strain gene regulatory elements near posttranslational-modifying enzymes have come underneath in placental animals ( Lowe et al. If you loved this short article and you would like to get additional facts about lawyer article kindly stop by our own webpage. , 2011 ). Here, we combined an efficient method for protein extraction from tissue samples with lysine-acetylated peptide immunoprecipitation and high-accuracy tandem mass spectrometric (MS/MS) measurements, which allowed us to generate an atlas of lysine acetylation sites in sixteen totally different rat tissues.

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